Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19904411.
- Also identified by DOI 10.1039/b913309g.
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Abstract
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.
Medical subject headings
- DNA
- Microfluidic Analytical Techniques