Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays.
basic_science · Level V
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- Record sourced from PubMed, PMID 19904412.
- Also identified by DOI 10.1039/b912646e.
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Abstract
We demonstrate ElectroWetting-On-Dielectric (EWOD) transport and SQUID gradiometer detection of magnetic nanoparticles (MNPs) suspended in a 2 microl de-ionized water droplet. This proof-of-concept methodology constitutes the first development step towards a highly sensitive magnetic immunoassay platform with SQUID readout and droplet-based sample handling. Magnetic AC-susceptibility measurements were performed on MNPs with a hydrodynamic diameter of 100 nm using a high-Tc dc Superconducting Quantum Interference Device (SQUID) gradiometer as detector. We observed that the signal amplitude per unit volume is 2.5 times higher for a 2 microl sample droplet compared to a 30 microl sample volume.
Medical subject headings
- Electrowetting
- Immunoassay
- Magnetics
- Microfluidic Analytical Techniques