High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis.
basic_science · Level V
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- Record sourced from PubMed, PMID 19915551.
- Also identified by DOI 10.1038/nbt.1589 and PMC identifier 2849652.
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Abstract
We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three bacteriophage promoters and three mammalian core promoters in a single experiment per promoter. The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology.
Medical subject headings
- Algorithms
- DNA
- Mutagenesis, Site-Directed
- Regulatory Elements, Transcriptional
- Sequence Analysis, DNA