Analysis of short tandem repeats by parallel DNA threading.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19915680.
- Also identified by DOI 10.1371/journal.pone.0007823 and PMC identifier 2773041.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the number of targeted STRs.
Medical subject headings
- DNA
- Tandem Repeat Sequences