Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
basic_science · Level V
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- Record sourced from PubMed, PMID 1992452.
- Also identified by PMC identifier 50943.
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Abstract
A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.
Medical subject headings
- RNA Polymerase I
- RNA, Ribosomal
- Saccharomyces cerevisiae
- Transcription, Genetic