Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.
basic_science · Level V
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- Record sourced from PubMed, PMID 19946276.
- Also identified by DOI 10.1038/nmeth.1404 and PMC identifier 2862482.
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Abstract
Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.
Medical subject headings
- Chromatin
- Chromatin Immunoprecipitation
- Sequence Analysis, DNA