Electron lambda-tomography.
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- Record sourced from PubMed, PMID 19955423.
- Also identified by DOI 10.1073/pnas.0906391106 and PMC identifier 2799867.
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Abstract
Filtered back-projection and weighted back-projection have long been the methods of choice within the electron microscopy community for reconstructing the structure of macromolecular assemblies from electron tomography data. Here, we describe electron lambda-tomography, a reconstruction method that enjoys the benefits of the above mentioned methods, namely speed and ease of implementation, but also addresses some of their shortcomings. In particular, compared to these standard methods, electron lambda-tomography is less sensitive to artifacts that come from structures outside the region that is being reconstructed, and it can sharpen boundaries.