High-throughput generation of selected reaction-monitoring assays for proteins and proteomes.
basic_science · Level V
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- Record sourced from PubMed, PMID 19966807.
- Also identified by DOI 10.1038/nmeth.1408.
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Abstract
Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases.
Medical subject headings
- Biological Assay
- High-Throughput Screening Assays
- Mass Spectrometry
- Peptide Library
- Proteins
- Proteome