Floxin, a resource for genetically engineering mouse ESCs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19966808.
- Also identified by DOI 10.1038/nmeth.1406 and PMC identifier 2895430.
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Abstract
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
Medical subject headings
- Embryonic Stem Cells
- Genetic Engineering