Single-gene transgenic mouse strains for reprogramming adult somatic cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20010831.
- Also identified by DOI 10.1038/nmeth.1410 and PMC identifier 3048025.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.
Medical subject headings
- Cellular Reprogramming
- Induced Pluripotent Stem Cells
- Transgenes