A reprogrammable mouse strain from gene-targeted embryonic stem cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20010832.
- Also identified by DOI 10.1038/nmeth.1409 and PMC identifier 3987893.
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Abstract
The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.
Medical subject headings
- Cellular Reprogramming
- Embryonic Stem Cells
- Gene Targeting
- Induced Pluripotent Stem Cells