Two-color, two-photon uncaging of glutamate and GABA.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20037590.
- Also identified by DOI 10.1038/nmeth.1413 and PMC identifier 4135702.
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Abstract
We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.
Medical subject headings
- Glutamic Acid
- Hippocampus
- Lighting
- Membrane Potentials
- Microscopy, Fluorescence, Multiphoton
- Pyramidal Cells
- gamma-Aminobutyric Acid