Immunological reactivity of blood from healthy humans to the rAls3p-N vaccine protein.
basic_science · Level V
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- Record sourced from PubMed, PMID 20039802.
- Also identified by DOI 10.1086/649901 and PMC identifier 2812804.
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Abstract
We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin G titers (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme-linked immunosorbent assay. rAls3p-N stimulated interferon gamma (IFN-gamma) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-gamma, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials.
Medical subject headings
- Antibodies, Fungal
- Candida
- Candidiasis
- Fungal Proteins
- Fungal Vaccines