Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 2006150.
- Also identified by PMC identifier 51180.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants. It should now be possible to systematically manipulate the trypanosome genome, directing predetermined mutations to virtually any chromosomal locus.
Medical subject headings
- Calmodulin
- Recombination, Genetic
- Transformation, Genetic
- Trypanosoma brucei brucei