Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20062050.
- Also identified by DOI 10.1038/nmeth.1414 and PMC identifier 2860480.
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Abstract
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.
Medical subject headings
- Chromosome Mapping
- Colonic Neoplasms
- DNA Methylation
- DNA, Neoplasm
- Genetic Testing