Counting single molecules in sub-nanolitre droplets.

Rane, T D; Puleo, C M; Liu, K J; Zhang, Y; Lee, A P; Wang, T H · Lab Chip · 2010

other · Level V

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Abstract

We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.

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