FRT-seq: amplification-free, strand-specific transcriptome sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20081834.
- Also identified by DOI 10.1038/nmeth.1417 and PMC identifier 2861772.
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Abstract
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
Medical subject headings
- Chromosome Mapping
- Fluorescence Resonance Energy Transfer
- Gene Expression Profiling
- Sequence Analysis, DNA
- Transcription Factors