Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20081836.
- Also identified by DOI 10.1038/nmeth.1415.
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Abstract
Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (>3.7 ns) and high quantum yields (>0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor.
Medical subject headings
- Bacterial Proteins
- Green Fluorescent Proteins
- Microscopy, Fluorescence
- Sequence Analysis, Protein