Molecular cloning and chromosomal mapping of a human galactoside-binding protein.
basic_science · Level V
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Abstract
A human galactoside-binding protein with an Mr of 31,000 was cloned from the human HT-1080 fibrosarcoma complementary DNA library. A partial complementary DNA clone containing the complete coding region was characterized and the deduced sequence encodes a polypeptide of 242 amino acids with the characteristics of a carbohydrate-binding protein. The gene coding for the human galactoside-binding protein was mapped to the chromosomal band 1p13. The deduced amino acid sequence of the human galactoside-binding protein revealed 95 residues at the amino terminus, homologous to the predicted amino acid sequence of the second exon of the human L-myc gene.
Medical subject headings
- Chromosome Banding
- Chromosomes, Human, Pair 1
- Hemagglutinins
- Nucleic Acid Hybridization
- RNA, Messenger
- Sequence Alignment
- Sequence Homology, Nucleic Acid