XDIA: improving on the label-free data-independent analysis.

Carvalho, Paulo C; Han, Xuemei; Xu, Tao; Cociorva, Daniel; Carvalho, Maria da Gloria; Barbosa, Valmir C; Yates, John R · Bioinformatics · 2010

basic_science · Level V

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Abstract

XDIA is a computational strategy for analyzing multiplexed spectra acquired using electron transfer dissociation and collision-activated dissociation; it significantly increases identified spectra (approximately 250%) and unique peptides (approximately 30%) when compared with the data-dependent ETCaD analysis on middle-down, single-phase shotgun proteomic analysis. Increasing identified spectra and peptides improves quantitation statistics confidence and protein coverage, respectively. The software and data produced in this work are freely available for academic use at http://fields.scripps.edu/XDIA CONTACT: paulo@pcarvalho.com Supplementary data are available at Bioinformatics online.

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