Detecting the conformation of individual proteins in live cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20118931.
- Also identified by DOI 10.1038/nmeth.1421 and PMC identifier 2844853.
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Abstract
We combined single-molecule fluorescence resonance energy transfer (smFRET) with single-particle tracking in live cells to detect the in vivo conformation of individual proteins. We site-specifically labeled recombinant SNARE proteins with a FRET donor and acceptor before microinjecting them into cultured cells. Individual proteins rapidly incorporated into folded complexes at the cell membrane, demonstrating the potential of this method to reveal dynamic interactions within cells.
Medical subject headings
- Protein Conformation
- SNARE Proteins