Tracking a molecular motor with a nanoscale optical encoder.

Wickersham, Charles E; Cash, Kevin J; Pfeil, Shawn H; Bruck, Irina; Kaplan, Daniel L; Plaxco, Kevin W; Lipman, Everett A · Nano Lett · 2010

basic_science · Level V

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Abstract

Optical encoders are commonly used in macroscopic machines to make precise measurements of distance and velocity by translating motion into a periodic signal. Here we show how Forster resonance energy transfer can be used to implement this technique at the single-molecule scale. We incorporate a series of acceptor dye molecules into self-assembling DNA, and the periodic signal resulting from unhindered motion of a donor-labeled molecular motor provides nanometer-scale resolution in milliseconds.

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