A nonviral minicircle vector for deriving human iPS cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 20139967.
- Also identified by DOI 10.1038/nmeth.1426 and PMC identifier 2892897.
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Abstract
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
Medical subject headings
- DNA, Circular
- Genetic Vectors
- Induced Pluripotent Stem Cells