ROCK inhibition facilitates tissue reconstitution from embryonic kidney cell suspensions.
Level V
Where this comes from
- Record sourced from PubMed, PMID 20150939.
- Also identified by DOI 10.1038/ki.2009.488.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Explanted embryonic kidney tissues, when placed in organ culture, recapitulate key aspects of nephron formation. Yet attempts to dissociate these tissues into single cells to make them more accessible for genetic manipulation and tissue recombination have been disappointing. Now it is reported that inhibitors of Rho-associated kinase (ROCK) facilitate tissue reconstitution and nephron differentiation from single-cell suspensions of the embryonic kidney. This finding illustrates an unexpected potential for self-organization in these cells and introduces a window of single-cell accessibility to the organ culture system.
Medical subject headings
- Kidney
- Regeneration
- rho-Associated Kinases