Electrokinetic concentration of DNA polymers in nanofluidic channels.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20151696.
- Also identified by DOI 10.1021/nl902228p.
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Abstract
DNA molecules can be concentrated in a narrow region of a nanochannel when driven electrokinetically in submillimolar salt solutions. Transport experiments and theoretical modeling reveal the interplay of electrophoresis, electro-osmosis, and the unique statistical properties of confined polymers that lead to DNA aggregation. A finite conductance through the bulk of the device also plays a crucial role by influencing the electric fields in the nanochannel. We build on this understanding by demonstrating how a nanofluidic device with integrated electrodes can preconcentrate DNA at selected locations and at physiological salt concentrations that are relevant to lab-on-a-chip applications.
Medical subject headings
- DNA
- Electrochemistry
- Microfluidic Analytical Techniques
- Micromanipulation
- Models, Chemical
- Nanostructures