Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging.

Bub, Gil; Tecza, Matthias; Helmes, Michiel; Lee, Peter; Kohl, Peter · Nat Methods · 2010

basic_science · Level V

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Abstract

We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.

Medical subject headings