Organelle-specific, rapid induction of molecular activities and membrane tethering.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20154678.
- Also identified by DOI 10.1038/nmeth.1428 and PMC identifier 2860863.
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Abstract
Using new chemically inducible dimerization probes, we generated a system to rapidly target proteins to individual intracellular organelles. Using this system, we activated Ras GTPase at distinct intracellular locations and induced tethering of membranes from two organelles, endoplasmic reticulum and mitochondria. Innovative techniques to rapidly perturb molecular activities and organelle-organelle communications at precise locations and timing will provide powerful strategies to dissect spatiotemporally complex biological processes.
Medical subject headings
- Cell Membrane
- Endoplasmic Reticulum
- Mitochondria
- Tacrolimus Binding Proteins