Fluorescent structural DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20158249.
- Also identified by DOI 10.1021/nl9039718 and PMC identifier 2843431.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.
Medical subject headings
- DNA
- Fluorescent Dyes
- Nanostructures
- Nanotechnology
- Nucleotides