Light-induced local heating for thermophoretic manipulation of DNA in polymer micro- and nanochannels.
basic_science · Level V
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- Record sourced from PubMed, PMID 20166745.
- Also identified by DOI 10.1021/nl903190q.
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Abstract
We present a method for making polymer chips with a narrow-band near-infrared absorber layer that enables light-induced local heating of liquids inside fluidic micro- and nanochannels fabricated by thermal imprint in polymethyl methacrylate. We have characterized the resulting liquid temperature profiles in microchannels using the temperature dependent fluorescence of the complex [Ru(bpy)(3)](2+). We demonstrate thermophoretic manipulation of individual YOYO-1 stained T4 DNA molecules inside micro- and nanochannels.
Medical subject headings
- DNA
- Electrophoresis
- Microfluidic Analytical Techniques
- Micromanipulation
- Nanostructures