Influence of genetic background and tissue types on global DNA methylation patterns.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20186319.
- Also identified by DOI 10.1371/journal.pone.0009355 and PMC identifier 2826396.
- Licence recorded as CC0.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Recent studies have shown a genetic influence on gene expression variation, chromatin, and DNA methylation. However, the effects of genetic background and tissue types on DNA methylation at the genome-wide level have not been characterized extensively. To study the effect of genetic background and tissue types on global DNA methylation, we performed DNA methylation analysis using the Affymetrix 500K SNP array on tumor, adjacent normal tissue, and blood DNA from 30 patients with esophageal squamous cell carcinoma (ESCC). The use of multiple tissues from 30 individuals allowed us to evaluate variation of DNA methylation states across tissues and individuals. Our results demonstrate that blood and esophageal tissues shared similar DNA methylation patterns within the same individual, suggesting an influence of genetic background on DNA methylation. Furthermore, we showed that tissue types are important contributors of DNA methylation states.
Medical subject headings
- Carcinoma, Squamous Cell
- DNA Methylation
- DNA, Neoplasm
- Esophageal Neoplasms