Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20208532.
- Also identified by DOI 10.1038/nmeth.1438 and PMC identifier 2850821.
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Abstract
We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.
Medical subject headings
- Integrases
- Toxoplasma
- Toxoplasmosis