Surface-patterned electrode bioreactor for electrical stimulation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20221556.
- Also identified by DOI 10.1039/b917743d and PMC identifier 3618471.
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Abstract
We present a microscale cell culture system with an interdigitated microarray of excimer-laser-ablated indium tin oxide electrodes for electrical stimulation of cultured cells. The system has been characterized in a range of geometeries and stimulation regimes via electrochemical impedance spectroscopy and used to culture primary cardiomyocytes and human adipose derived stem cells. Over 6 days of culture with electrical stimulation (2 ms duration, 1 Hz, 180 microm wide electrodes with 200 microm spacing), both cell types exhibited enhanced proliferation, elongation and alignment, and adipose derived stem cells exhibited higher numbers of Connexin-43-composed gap junctions.
Medical subject headings
- Bioreactors
- Cell Culture Techniques
- Electric Stimulation
- Electrodes
- Microfluidics
- Myocytes, Cardiac