Direct determination of molecular haplotypes by chromosome microdissection.

Ma, Li; Xiao, Yan; Huang, Hui; Wang, Qingwei; Rao, Weinian; Feng, Yue; Zhang, Kui; Song, Qing · Nat Methods · 2010

basic_science · Level V

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Abstract

Direct observation of haplotypes is still technically challenging. Here we report a method for the determination of haplotypes through chromosome microdissection. We determined human long-range chromosomal haplotypes with more than 98.85% accuracy at 24,245 genome-wide heterozygous single-nucleotide polymorphism (SNP) loci.

Medical subject headings