Super-SILAC mix for quantitative proteomics of human tumor tissue.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20364148.
- Also identified by DOI 10.1038/nmeth.1446.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.
Medical subject headings
- Breast Neoplasms
- Isotope Labeling
- Neoplasm Proteins
- Proteomics