CO I barcoding reveals new clades and radiation patterns of Indo-Pacific sponges of the family Irciniidae (Demospongiae: Dictyoceratida).
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20376349.
- Also identified by DOI 10.1371/journal.pone.0009950 and PMC identifier 2848591.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
BACKGROUND: DNA barcoding is a promising tool to facilitate a rapid and unambiguous identification of sponge species. Demosponges of the order Dictyoceratida are particularly challenging to identify, but are of ecological as well as biochemical importance. METHODOLOGY/PRINCIPAL FINDINGS: Here we apply DNA barcoding with the standard CO1-barcoding marker on selected Indo-Pacific specimens of two genera, Ircinia and Psammocinia of the family Irciniidae. We show that the CO1 marker identifies several species new to science, reveals separate radiation patterns of deep-sea Ircinia sponges and indicates dispersal patterns of Psammocinia species. However, some species cannot be unambiguously barcoded by solely this marker due to low evolutionary rates. CONCLUSIONS/SIGNIFICANCE: We support previous suggestions for a combination of the standard CO1 fragment with an additional fragment for sponge DNA barcoding.
Medical subject headings
- DNA
- Electron Transport Complex IV
- Electronic Data Processing
- Porifera