Using buoyant mass to measure the growth of single cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20383132.
- Also identified by DOI 10.1038/nmeth.1452 and PMC identifier 2862099.
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Abstract
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
Medical subject headings
- Cell Enlargement
- Microfluidic Analytical Techniques