Targeted gene deletions in C. elegans using transposon excision.
basic_science · Level V
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- Record sourced from PubMed, PMID 20418868.
- Also identified by DOI 10.1038/nmeth.1454 and PMC identifier 2878396.
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Abstract
We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.
Medical subject headings
- Caenorhabditis elegans
- DNA Transposable Elements
- Gene Deletion