Generating knockout rats by transposon mutagenesis in spermatogonial stem cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20473302.
- Also identified by DOI 10.1038/nmeth.1461 and PMC identifier 2886193.
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Abstract
Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.
Medical subject headings
- DNA Transposable Elements
- Gene Knockout Techniques
- Mutagenesis
- Rats
- Spermatogonia
- Stem Cells