Synthetic polymer coatings for long-term growth of human embryonic stem cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20512122.
- Also identified by DOI 10.1038/nbt.1631 and PMC identifier 3471651.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.
Medical subject headings
- Cell Culture Techniques
- Coated Materials, Biocompatible
- Embryonic Stem Cells
- Polymers