Estimating prion concentration in fluids and tissues by quantitative PMCA.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20512142.
- Also identified by DOI 10.1038/nmeth.1465 and PMC identifier 4049222.
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Abstract
Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.
Medical subject headings
- PrPSc Proteins
- Scrapie