Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels.

Westerlund, Fredrik; Persson, Fredrik; Kristensen, Anders; Tegenfeldt, Jonas O · Lab Chip · 2010

basic_science · Level V

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Abstract

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

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