Rationally improving LOV domain-based photoswitches.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20562867.
- Also identified by DOI 10.1038/nmeth.1473 and PMC identifier 2914111.
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Abstract
Genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. Here we report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of Avena sativa phototropin 1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches.
Medical subject headings
- Light
- Photochemistry
- Phototropins
- Protein Engineering