Validation of 11 kGy as a radiation sterilization dose for frozen bone allografts.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20570097.
- Also identified by DOI 10.1016/j.arth.2010.03.032.
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Abstract
A radiation sterilization dose (RSD) of 25 kGy is deleterious to bone allografts. This study aimed to establish a lower RSD for bone allografts using method 1 of International Standard Organisation 11137.2:2006. This provides a database to select an RSD corresponding to an allograft's bioburden, given that the bioburden's gamma resistance is equal to or less than the standard. This can be verified by irradiating 100 allografts at a dose selected to provide a sterility assurance level of 10(-2). The bioburden of our allografts was 0, which prescribed a verification dose of 1.3 kGy. After irradiating 100 allografts, sterility tests returned no positive cultures. We therefore validated an RSD of 11 kGy for allografts with that bioburden. According to the standard, this RSD provides a sterility assurance level of 10(-6) for bone allografts.
Medical subject headings
- Bone Transplantation
- Cryopreservation
- Radiation Dosage
- Sterilization