Simultaneous intracellular chloride and pH measurements using a GFP-based sensor.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20581829.
- Also identified by DOI 10.1038/nmeth.1471.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.
Medical subject headings
- Chlorides
- Green Fluorescent Proteins