A photoactivatable marker protein for pulse-chase imaging with superresolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20601949.
- Also identified by DOI 10.1038/nmeth.1477.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).
Medical subject headings
- Luminescent Proteins
- Microscopy
- Radioisotopes