Impaired T-cell receptor activation in IL-1 receptor-associated kinase-4-deficient patients.

McDonald, Douglas R; Goldman, Frederick; Gomez-Duarte, Oscar D; Issekutz, Andrew C; Kumararatne, Dinakantha S; Doffinger, Rainer; Geha, Raif S · J Allergy Clin Immunol · 2010

case_control · Level III

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Abstract

IL-1 receptor-associated kinase 4 (IRAK-4) is an effector of the Toll-like receptor and IL-1 receptor pathways that plays a critical role in innate immune responses. The role of IRAK-4 in adaptive immune functions in human subjects is incompletely understood. We sought to evaluate T-cell function in IRAK-4 deficient patients. We compared upregulation of CD25 and CD69 on T cells and production of IL-2, IL-6, and IFN-gamma after stimulation of PBMCs from 4 IRAK-4-deficient patients and healthy control subjects with anti-CD3 and anti-CD28. Upregulation of CD25 and CD69 on T cells and production of IL-6 and IFN-gamma, but not IL-2, was significantly reduced in IRAK-4-deficient patients. IRAK-4-deficient patients have defects in T-cell activation.

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