Single-allele analysis of transcription kinetics in living mammalian cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20639867.
- Also identified by DOI 10.1038/nmeth.1482.
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Abstract
We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.
Medical subject headings
- Alleles
- Transcription, Genetic