High-throughput in vivo vertebrate screening.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20639868.
- Also identified by DOI 10.1038/nmeth.1481 and PMC identifier 2941625.
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Abstract
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
Medical subject headings
- Axons
- Mass Screening
- Microscopy, Confocal
- Retina