Live-cell super-resolution imaging with trimethoprim conjugates.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 20693998.
- Also identified by DOI 10.1038/nmeth.1489.
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Abstract
The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at approximately 20 nm resolution.
Medical subject headings
- Histones
- Microscopy, Fluorescence
- Trimethoprim