MASiVE: Mapping and Analysis of Sirevirus Elements in plant genome sequences.

Darzentas, N; Bousios, A; Apostolidou, V; Tsaftaris, A S · Bioinformatics · 2010

basic_science · Level V

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Abstract

We present MASiVE, an expertly built tool for the large-scale, yet sensitive and highly accurate, discovery, preliminary analysis and insertion age estimation of intact Sirevirus LTR-retrotransposons in plant genomic sequences. Validation was based on the recently available and annotated large maize chromosome one. Results show a considerable improvement in the annotation of Sireviruses, and support our approach as an important addition to the bioinformatics toolbox of plant biologists. PERL source code and essential files are available online at http://bat.ina.certh.gr/tools/masive/. The freely available Vmatch, LTRharvest, Wise2, and MAFFT algorithms are required.

Medical subject headings